Description
Size
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.
The LigaFast™ System is Blue/White Cloning Qualified, providing a higher level of quality control for enzymes used in cloning applications.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
Protocols
Complete Protocol
Item | Part # | Size |
---|---|---|
2X Rapid Ligation Buffer | C671A | 1 × 200μl |
T4 DNA Ligase | M180A | 1 × 100u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Item | Part # | Size |
---|---|---|
2X Rapid Ligation Buffer | C671B | 2 × 500μl |
T4 DNA Ligase | M180B | 1 × 500u |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.