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GoTaq® Flexi DNA Polymerase

GoTaq® Flexi DNA Polymerase

Optimize Enzyme and Magnesium Concentrations in Your PCR

  • Mg-free reaction buffers for ease of optimization
  • Direct-to-gel convenience
  • Supplied at a concentration of 5u/μl
  • Supplied at a concentration of 5u/μl

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Description

Size

Catalog number selected: M8291

Catalog number selected:  M8295

Catalog number selected: M8296

Catalog number selected: M8297

Catalog number selected: M8298

High-Performance Hot Start Taq with Convenient Room-Temperature PCR Setup

Maximum Flexibility, Control and Convenience for Optimizing PCR

GoTaq® Flexi DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq. The supplied 5X Green and Colorless Flexi Reaction Buffers do not contain magnesium. A separate tube of 25mM MgCl2 is included, giving you the option to titrate Mg2+concentration in your reactions. MgCl2 Solution is also available separately.

GoTaq® products streamline your PCR workflow. You can directly substitute GoTaq® Flexi DNA Polymerase, with either Colorless or Green Reaction Buffer, in your current PCR application without altering your cycling conditions. Green GoTaq® Reaction Buffer serves as both reaction buffer and gel-loading solution, allowing you to load reactions directly onto a gel for efficient analysis. GoTaq® products are compatible with PCR enhancers, such as betaine and DMSO, which do not affect the color or characteristics of the GoTaq® Green Reaction Buffer.

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Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Protocols

Complete Protocol

GoTaq® Flexi DNA Polymerase (M829) Protocol

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Specifications

What’s in the box?

  Item Catalog number selected: M8291 Part # Size Concentration Available Separately
MgCl2 A351B 1 × 750μl 25mM View Product
GoTaq® Flexi DNA Polymerase M829A 1 × 100u
5X Colorless GoTaq® Flexi Buffer M890A 1 × 1ml View Product
5X Green GoTaq® Flexi Buffer M891A 1 × 1ml View Product

SDS For M8291

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

  Item Catalog number selected: M8295 Part # Size Concentration Available Separately
MgCl2 A351H 3 × 1.2ml 25mM View Product
GoTaq® Flexi DNA Polymerase M829B 1 × 500u
5X Colorless GoTaq® Flexi Buffer M890A 4 × 1ml View Product
5X Green GoTaq® Flexi Buffer M891A 4 × 1ml View Product

SDS For M8295

Download SDS

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

  Item Catalog number selected: M8296 Part # Size Concentration Available Separately
MgCl2 A351H 15 × 1.2ml 25mM View Product
GoTaq® Flexi DNA Polymerase M829B 5 × 500u
5X Colorless GoTaq® Flexi Buffer M890A 20 × 1ml View Product
5X Green GoTaq® Flexi Buffer M891A 20 × 1ml View Product

SDS For M8296

Download SDS

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

  Item Catalog number selected: M8297 Part # Size Concentration Available Separately
MgCl2 A351H 30 × 1.2ml 25mM View Product
GoTaq® Flexi DNA Polymerase M829B 10 × 500u
5X Colorless GoTaq® Flexi Buffer M890A 40 × 1ml View Product
5X Green GoTaq® Flexi Buffer M891A 40 × 1ml View Product

SDS For M8297

Download SDS

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

  Item Catalog number selected: M8298 Part # Size Concentration Available Separately
MgCl2 A351H 60 × 1.2ml 25mM View Product
GoTaq® Flexi DNA Polymerase M829B 20 × 500u
5X Colorless GoTaq® Flexi Buffer M890A 80 × 1ml View Product
5X Green GoTaq® Flexi Buffer M891A 80 × 1ml View Product

SDS For M8298

Download SDS

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.