Description
GoTaq® G2 Flexi: Freedom to Optimize Mg Concentration
GoTaq® G2 Flexi DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. GoTaq® G2 Flexi DNA Polymerase is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity.
GoTaq® G2 Flexi DNA Polymerase is supplied with Mg-free 5X Green GoTaq® Flexi Reaction Buffer and 5X Colorless GoTaq® Flexi Reaction Buffer to facilitate Mg2+ titrations for PCR optimization. The 5X Green GoTaq® Reaction Buffer has two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The buffer also includes a compound that increases sample density so that samples can be loaded directly onto gels without the need for loading dye. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
GoTaq® G2 DNA Polymerase consistently produces high yields across multiple targets while competitors produce highly variable results.

Protocols
Complete Protocol
What’s in the box?
Item Catalog number selected: M7801 | Part # | Size | Concentration | Available Separately |
---|---|---|---|---|
MgCl2 | A351B | 1 × 750μl | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase | M780A | 1 × 100u | ||
5X Colorless GoTaq® Flexi Buffer | M890A | 1 × 1ml | View Product | |
5X Green GoTaq® Flexi Buffer | M891A | 1 × 1ml | View Product |
SDS For M7801
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions

What’s in the box?
Item Catalog number selected: M7805 | Part # | Size | Concentration | Available Separately |
---|---|---|---|---|
MgCl2 | A351H | 3 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase | M780B | 1 × 500u | ||
5X Colorless GoTaq® Flexi Buffer | M890A | 4 × 1ml | View Product | |
5X Green GoTaq® Flexi Buffer | M891A | 4 × 1ml | View Product |
SDS For M7805
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions

What’s in the box?
Item Catalog number selected: M7806 | Part # | Size | Concentration | Available Separately |
---|---|---|---|---|
MgCl2 | A351H | 15 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase | M780B | 5 × 500u | ||
5X Colorless GoTaq® Flexi Buffer | M890A | 20 × 1ml | View Product | |
5X Green GoTaq® Flexi Buffer | M891A | 20 × 1ml | View Product |
SDS For M7806
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions

What’s in the box?
Item Catalog number selected: M7808 | Part # | Size | Concentration | Available Separately |
---|---|---|---|---|
MgCl2 | A351H | 60 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase | M780B | 20 × 500u | ||
5X Colorless GoTaq® Flexi Buffer | M890A | 80 × 1ml | View Product | |
5X Green GoTaq® Flexi Buffer | M891A | 80 × 1ml | View Product |
SDS For M7808
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions

Patent and Disclaimers
Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.