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Set of dATP, dCTP, dGTP, dUTP

Set of dATP, dCTP, dGTP, dUTP

High-Quality dNTPs, Including dUTP

  • Highly pure (≥99% triphosphate)
  • Each dNTP supplied at 100mM in water for ease-of-use
  • Incorporate dUTP into PCR products and treat with uracil-N-glycosylase to inhibit subsequent amplifications

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Description

Size

Catalog number selected: U1335

Catalog number selected:  U1245

Ready to Use Solution of Deoxynucleotide Triphosphates

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing, cloning and genotyping. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.

dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.

  1. Longo, M.C. et al. (1990) Gene 93, 125–8.
  2. Erlich, H.A. et al. (1991) Science 252, 1643–51.

Specifications

What’s in the box?

Item/ Catalog number selected: U1335 Part # Size Concentration
dNTP Mix U151B 1 × 1,000μl 10mM
dATP U120B 1 × 100μl 100mM
dATP U120B 1 × 100μl 100mM
dATP U120B 1 × 100μl 100mM

SDS For U1335

Download SDS

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

Item/ Catalog number selected: U1245 Part # Size Concentration
dUTP U119A 1 × 400μl 100mM
dATP U120A 1 × 400μl 100mM
dGTP U121A 1 × 400μl 100mM
dCTP U122A 1 × 400μl 100mM

SDS For U1245

Download SDS

 

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions