The TeloPrime Full-Length cDNA Amplification Kit V2 is an all-in-one protocol for generating full-length cDNA from capped and polyadenylated RNAs. cDNA generated with TeloPrime is suitable for various downstream applications such as e.g., NGS library generation (incl. PacBio), probes, RACE, and cloning.
Features and Application:
- NGS library generation (including PacBio)
- generation of probes
- Reagent Box
- Buffers and Purification Columns
Full-Length cDNA Synthesis
Based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, it is highly selective for full-length RNA molecules that are both capped and polyadenylated.
For Multiple Downstream Applications
The full-length cDNA products can be used for various downstream applications such as NGS, RACE, cloning, microarray probes, and normalization. It enables the detection and correct quantification of splice variants and their true transcription start- and end-sites, in both short and long mRNA molecules. For further full-length or gene-specific PCRs, Lexogen offers a TeloPrime PCR Add-on Kit V2 (Cat.No.018.16) containing 16 rxn. This kit contains the PCR Forward and Reverse Primer separately,hence they can be alternatively substituted with the gene-specific primer of interest.
Exceptional Elimination of cDNA from Degraded RNA
In an mRNA-Seq experiment, mRNAs were tagged at their 5’ end using TeloPrime, Template-Switch (TS) or Oligo Capping (OC). Transcript start sites (TSS) were mapped to the mouse genome. The accumulated TSS read coverage is plotted versus the normalized annotated transcript length to show relative TSS mapping for the top 500 expressed genes.
TeloPrime Full-Length cDNA Amplification Kit V2
Product Flyer – Release of TeloPrime V2 (20.12.2018)
013UG022V0200 – User Guide – update 22.11.2018
|Catalog Nr.||Product Name|
|013.08||TeloPrime Full-Length cDNA Amplification Kit, 8 preps|
|013.24||TeloPrime Full-Length cDNA Amplification Kit, 24 preps|