Description
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase is provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP.
Applications
- 3´ end-labeling of RNA.
- Circularization of RNA and DNA oligonucleotides.
- Cloning full-length cDNA.
References
- Silber, R. et al. (1972) Proc. Natl. Acad. Sci. USA 69, 3009–13.
- England, T. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 4839–42.
- Uhlenbeck, O.C. and Gumport, R.I. (1982) The Enzymes 158, 31.
- Brennan, C.A. et al. (1983) Meth. Enzymol. 100, 38–52.
- Schaefer, B.C. (1995) Anal. Biochem. 227, 255–73.
Storage Buffer: 10mM Tris (pH 7.5 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.1% Tween® 20.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease.
Unit Definition: One unit catalyzes the formation of 1nmol of [5´-32P]rA14–20 into a phosphatase-resistant form in 30 minutes at 37°C in a total volume of 30μl. The reaction conditions are: 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 5mM DTT, 1mM ATP and 10μM [5´-32P]rA14–20.
Protocols
What’s in the box?
Size: 500u
| Item | Part # | Size |
|---|---|---|
| T4 RNA Ligase | M105A | 1 × 500u |
| T4 RNA Ligase 10X Buffer | M107A | 1 × 500μl |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
