Description
Hot Start Taq with Superior Performance, Improved Yield, Greater Sensitivity
GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.
GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products. In some cases, hot-start PCR may improve yields.
GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
GoTaq® G2 Hot Start Taq exhibits higher yields and greater detection sensitivity.
The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using GoTaq® G2 Hot Start Taq with Green (G) and Colorless (C) buffers and the leading competitors (L1, R, Q, Q+, L2 and L3) according to manufacturers’ recommendations.
Protocols
What’s in the box?
| Item Catalog number selected: M7401 | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
| MgCl2 | A351B | 1 × 750μl | 25mM | View Product |
| GoTaq® G2 Hot Start Polymerase | M740A | 1 × 100u | ||
| 5X Colorless GoTaq® Flexi Buffer | M890A | 1 × 1ml | View Product | |
| 5X Green GoTaq® Flexi Buffer | M891A | 1 × 1ml | View Product |
SDS For M7401
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures
Storage Conditions
What’s in the box?
| Item Catalog number selected: M7405 | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
| MgCl2 | A351H | 3 × 1.2ml | 25mM | View Product |
| GoTaq® G2 Hot Start Polymerase | M740B | 1 × 500u | ||
| 5X Colorless GoTaq® Flexi Buffer | M890A | 4 × 1ml | View Product | |
| 5X Green GoTaq® Flexi Buffer | M891A | 4 × 1ml | View Product |
SDS For M7405
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item Catalog number selected: M7406 | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
| MgCl2 | A351H | 15 × 1.2ml | 25mM | View Product |
| GoTaq® G2 Hot Start Polymerase | M740B | 5 × 500u | ||
| 5X Colorless GoTaq® Flexi Buffer | M890A | 20 × 1ml | View Product | |
| 5X Green GoTaq® Flexi Buffer | M891A | 20 × 1ml | View Product |
SDS For M74016
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item Catalog number selected: M7408 | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
| MgCl2 | A351H | 60 × 1.2ml | 25mM | View Product |
| GoTaq® G2 Hot Start Polymerase | M740B | 20 × 500u | ||
| 5X Colorless GoTaq® Flexi Buffer | M890A | 80 × 1ml | View Product | |
| 5X Green GoTaq® Flexi Buffer | M891A | 80 × 1ml | View Product |
SDS For M7408
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patent and Disclaimers
Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only
