Description
Size
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.
Reference
- Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.
Note: Units listed are Weiss units.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
Protocols
What’s in the box?
concentration: 1–3u/µl
Size: 100u (Weiss units)
| Item / Catalog no: M1801 | Part # | Size | Available Separately |
| T4 DNA Ligase 10X Buffer | C126B | 1 × 200μl | View Product |
| T4 DNA Ligase | M180A | 1 × 100u |
SDS
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
concentration: 1–3u/µl
Size: 500u (Weiss units)
| Item / Catalog no: M1804 | Part # | Size | Available Separately |
| T4 DNA Ligase 10X Buffer | C126A | 2 × 500μl | View Product |
| T4 DNA Ligase | M180B | 1 × 500u |
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
concentration: 10–20u/µl
Size: 500u (Weiss units)
| Item / Catalog no: M1794 | Part # | Size | Available Separately |
| T4 DNA Ligase 10X Buffer | C126B | 1 × 200μl | View Product |
| T4 DNA Ligase (HC) | M179A | 1 × 500u |
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
