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SV 96 Total RNA Isolation System

SV 96 Total RNA Isolation System

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Description

Size

Manual or High-Throughput RNA Purification. No Phenol, No Ethanol, No Fuss.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.

Protocols are available for Beckman Coulter and PerkinElmer instruments.

  • Tested to ensure that purified RNA performs optimally in RT-PCR.
  • Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing.

The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

For automated total RNA purification using the Biomek® 2000 Laboratory Automation Workstation, the Vacuum Manifold (Beckman Coulter Part# 609670) and Collar (Beckman Coulter Part# 609597) are required. for additional information on automated RNA purification.

Protocols

Complete Protocol

SV 96 Total RNA Isolation System Technical Bulletin

 

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Quick Protocols

SV 96 Total RNA Isolation System Quick Protocol FB075

 

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Specifications

What’s in the box?

Item - Catalog number: Z3500 Part # Size Available Separately
Binding Plate A227A 1 × 1 plate View Product
Elution Plate A228A 1 × 1 plate
Plate Sealers A230A 1 × 3 each
Nuclease-Free Water P119E 1 × 13ml View Product
RNA Lysis Buffer (RLA) Z3051 1 × 50ml View Product
RNA Wash Solution (RWA) Z3091 1 × 58.8ml
DNase Stop Solution (DSA) Z312C 1 × 13.25ml
Yellow Core Buffer Z317A 1 × 2.5ml
MnCl2, 0.09M Z318B 1 × 750μl
DNase I (lyophilized) Z358A 1 × 1 vial
Beta-Mercaptoethanol Z559A 1 × 900μl

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations

What’s in the box?

Item - Catalog number: Z3505 Part # Size Available Separately
Binding Plate A227A 5 × 1 plate View Product
Plate Sealers A230A 2 × 3 each
RNA Lysis Buffer (RLA) Z3051 2 × 50ml View Product
RNA Wash Solution (RWA) Z3091 5 × 58.8ml
Beta-Mercaptoethanol Z559A 2 × 900μl

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.