Description
Cut Site:
AGTâ–ĽACT
TCAâ–˛TGA
Incubation Conditions: Buffer K. 37°C.
Source: Streptomyces caespitosus.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | K | MULTI-CORE™ |
| <10% | 100%* | 50–75% | 75–100% | 100% | 10–25%* |
| *Buffer B and MULTI-CORE™ Buffer are not recommended due to potential star activity. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 5 | 5 | 0 | 1 | 0 | 1 |
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer K 10X Buffer | R010A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| ScaI | R621A | 1 Ă— 1,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
