Description
Cut Site:
CC GC▼GG
GG▲CG CC
Incubation Conditions: Buffer C. 37°C.
Source: Streptomyces achromogenes.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 100%* | 50–75% | 100% | 50–75% | <10% | |
| *Buffer A is not recommended for use with supercoiled plasmid due to incomplete cutting. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 4 | 33 | 1 | 0 | 0 | 0 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer C 10X Buffer | R003A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| SacII | R622A | 1 × 500u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
