Description
Cut Site:
GÂ Â AGCTâ–ĽC
Câ–˛TCGA Â G
Note: SacI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, EcoICRI.
Incubation Conditions: Buffer J. 37°C.
Source: Streptomyces achromogenes.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | J | MULTI-CORE™ |
| 75–100% | 25–50% | 25–50% | <10% | 100% | 100% |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 2 | 16 | 0 | 1 | 1 | 0 |
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
You are viewing:Â R6061 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer J 10X Buffer | R009A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| SacI | R606A | 1 Ă— 1,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6065 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer J 10X Buffer | R009A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 Ă— 750ÎĽl | 10mg/ml | View Product |
| SacI | R606E | 1 Ă— 5,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
