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SacI

Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

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Description

10u/μl

Catalog number selected: R6061

1,000u

Catalog number selected: R6061

5,000u

Catalog number selected: R6065

Cut Site:

G  AGCTC
CTCGA  G

Note: SacI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, EcoICRI.

Incubation Conditions: Buffer J. 37°C.

Source: Streptomyces achromogenes.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D J MULTI-CORE™
75–100% 25–50% 25–50% <10% 100% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
2 16 0 1 1 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

You are viewing: R6061 Change Configuration
Item Part # Size Concentration Available Separately
Buffer J 10X Buffer R009A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
SacI R606A 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6065 Change Configuration
Item Part # Size Concentration Available Separately
Buffer J 10X Buffer R009A 1 × 1ml
Bovine Serum Albumin, Acetylated R396E 1 × 750μl 10mg/ml View Product
SacI R606E 1 × 5,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions