Description
Intact, Amplifiable DNA from FFPE Tissue
Higher quality DNA from FFPE samples
ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.
The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.
The protocol is complete in 2.5 hours with minimal hands-on time.
Purified FFPE DNA is Compatible with Sensitive Assays
Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641).
Simple Protocol
Protocols
Complete Protocol
ReliaPrep™ FFPE gDNA Miniprep System Technical Manual
Quick Protocols
ReliaPrep™ FFPE gDNA Miniprep System Quick Protocol FB124
What’s in the box?
| Item Catlog number: A2351 | Part # | Size | Concentration | Available Separately |
| Collection Tubes | A130C | 1 × 10/pk | ||
| Mineral Oil | A244A | 1 × 5ml | ||
| ReliaPrep™ FFPE Binding Columns | A247A | 1 × 10/pk | ||
| Proteinase K (PK) Solution | A505A | 1 × 250μl | View Product | |
| RNase A Solution | A797D | 1 × 200μl | 4mg/ml | View Product |
| Elution Buffer | A828C | 1 × 15ml | View Product | |
| Lysis Buffer (LBA) | Z101A | 2 × 1ml | ||
| BL Buffer | Z103A | 1 × 3.25ml | ||
| Wash Solution | Z104A | 1 × 3ml | ||
| Blue Dye | MC122A | 1 × 100μl |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item Catlog number: A2352 | Part # | Size | Concentration | Available Separately |
| Collection Tubes | A130A | 2 × 50/pk | ||
| Mineral Oil | A244B | 1 × 50ml | ||
| ReliaPrep™ FFPE Binding Columns | A247B | 2 × 50/pk | ||
| Proteinase K (PK) Solution | A505B | 2 × 1.1ml | View Product | |
| RNase A Solution | A797C | 1 × 1ml | 4mg/ml | View Product |
| Elution Buffer | A828C | 1 × 15ml | View Product | |
| Lysis Buffer (LBA) | Z101B | 1 × 30ml | ||
| BL Buffer | Z103B | 1 × 32.5ml | ||
| Wash Solution | Z104B | 1 × 30ml | ||
| Blue Dye | MC122A | 1 × 100μl |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
