Description
Sensitive Quantitation of Single-Stranded DNA
Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.
The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful many molecular biology research applications, including studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.
The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg), so you save more of your sample for downstream assays.
Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems
For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.
The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent
| Sample* | Assay | |
| QuantiFluor® ssDNA System | 0.2–400ng/µl | 0.2–400ng |
| Quant-iT™ OliGreen | 0.2–400ng/µl | 0.2–400ng |
| Qubit® ssDNA | 1–200ng/µl | 1–200ng |
| NanoDrop® 2000 Spectrophotometer | 2–12,000ng/µl | 2–12,000ng |
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
ssDNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
