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pSP72 Vector

pSP72 Vector

Standard Cloning Vector Used to Transcribe RNA In Vitro

  • Versatile vector that can be used for standard cloning and in vitro transcription
  • SP6 and T7 RNA polymerase promoters flank the multiple cloning region
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

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Description

Size: 20µg
Catalog number: P2191

The pSP72 Vector is a standard cloning vector that can be used for transcription of RNA in vitro. The pSP72 Vector contains the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning site region.

Reference

  1. Krieg, P.A. and Melton, D.A. (1987) Meth. Enzymol. 155, 397–415.
0298VA05_2A-W

pSP72 Vector GenBank® Accession Number X65332 and vector sequence text file.

Protocols

Complete Protocol

pSP72 Vector Technical Bulletin


Specifications

What’s in the box?
Size: 20µg

Item Part # Size
pSP72 Vector P219A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB