Description
Cut Site:
G GTAC▼C
C▲CATG G
Usage Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- KpnI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, Acc65I.
- KpnI is not sensitive to overlapping dcm methylation, unlike its neoschizomer, Acc65I.
Incubation Conditions: Buffer J. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 200mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | J | MULTI-CORE™ |
| 100%* | 25–50% | 25–50% | <10% | 100% | 75–100% |
| *Buffer A is not recommended due to potential star activity. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 2 | 8 | 0 | 1 | 1 | 0 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6341 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer J 10X Buffer | R009A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| KpnI | R634A | 1 × 2,500u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6345 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer J 10X Buffer | R009A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| KpnI | R634G | 1 × 10,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
