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HB101 Competent Cells

HB101 Competent Cells

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Description

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1ml

(5 x 200µl)

Catalog number selected: L2011

1ml

(20 x 50µl)

Catalog number selected: L2015

HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

HB101 Competent Cells are prepared according to a modified procedure of Hanahan.

HB101 Genotype: F–, thi-1, hsdS20 (rB, mB), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Complete Protocol

E. coli Competent Cells Technical Bulletin

Quick Protocol

E Coli Competent Cells Quick Protocol FB035

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Specifications

What’s in the box?

You are viewing:  L2011  Change Configuration
Item Part # Size
Competent Cells Control DNA L107A 1 × 30μl
HB101 Competent Cells, High Efficiency L201A 5 × 200μl

SDS

 

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing:  L2015 Change Configuration
Item Part # Size
Single-Use HB101 Competent Cells, >108cfu/µg L201B 20 × 50μl

SDS

 

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions