Description
Cut Site:
G▼AATT C
C TTAA▲G
Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/μg, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.
Incubation Conditions: Buffer H. 37°C.
Source: Escherichia coli RY 13.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | H | MULTI-CORE™ |
| 25–50% | 50–75% | 50–75% | 50–75% | 100% | 100%* |
| *MULTI-CORE™ Buffer is not recommended due to potential star activity. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 5 | 5 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6011 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer H 10X Buffer | R008A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| EcoRI | R601A | 1 × 5,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6025 & R6045 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer H 10X Buffer | R008A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| EcoRI | R601J | 1 × 15,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6025 & R4014 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer H 10X Buffer | R008A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396F | 1 × 1,250μl | 10mg/ml | View Product |
| EcoRI (HC) | R401A | 1 × 25,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
