Description
Catalog number selected: A8882
Catalog number selected: A8880
Direct Amplification for Rapid PCR Results
The XpressAmp™ Direct Amplification Reagents provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.
How the XpressAmp™ Direct Amplification Reagents Work
Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.
Lyse Sample
Mix sample and XpressAmp™ Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.
Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp™ Solution.
Direct PCR Amplification Benefits and Challenges
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with RSV A and Influenza B (Hong Kong) virus reconstituted from Helix Elite™ Inactivated Standard, Inactivated Influenza A/B and Respiratory Syncytial Virus. This high-level virus sample (1 × 103 copies/μl) was diluted 1:10 and 1:100 in VTM to create the medium- and low-level virus samples. In parallel, two users created sample lysates from the spiked VTM samples using the XpressAmp™ Direct Amplification Reagents. Both users then detected the presence of RSV A and Influenza B by RT-qPCR using GoTaq® Probe 1-Step qPCR System (Cat.# A6121) supplemented with the XpressAmp™ Solution. N=6.
Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp™ Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp™ Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.
Saliva Direct Protocol for SARS-CoV-2 Surveillance Testing
Evidence of Viral Inactivation by XpressAmp™ Lysis Buffer Video
What’s in the box?
| Item/ Catalog number selected: A8882 | Part # | Size |
|---|---|---|
| XpressAmp™ Lysis Buffer | A886A | 1 × 15ml |
| XpressAmp™ Solution | A887A | 1 × 1.5ml |
| 1-Thioglycerol | A208A | 2 × 75μl |
SDS For A8882
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
| Item/ Catalog number selected: A8880 | Part # | Size |
|---|---|---|
| XpressAmp™ Lysis Buffer | A886B | 1 × 200ml |
| XpressAmp™ Solution | A887B | 1 × 18ml |
| 1-Thioglycerol | A208C | 2 × 1ml |
SDS For A8880
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
