Description
Catalog number selected: A8882
Catalog number selected: A8880
Direct Amplification for Rapid PCR Results
The XpressAmp™ Direct Amplification Reagents provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.
How the XpressAmp™ Direct Amplification Reagents Work
Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.
Lyse Sample
Mix sample and XpressAmp™ Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.
Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp™ Solution.
Direct PCR Amplification Benefits and Challenges
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with RSV A and Influenza B (Hong Kong) virus reconstituted from Helix Elite™ Inactivated Standard, Inactivated Influenza A/B and Respiratory Syncytial Virus. This high-level virus sample (1 × 103 copies/μl) was diluted 1:10 and 1:100 in VTM to create the medium- and low-level virus samples. In parallel, two users created sample lysates from the spiked VTM samples using the XpressAmp™ Direct Amplification Reagents. Both users then detected the presence of RSV A and Influenza B by RT-qPCR using GoTaq® Probe 1-Step qPCR System (Cat.# A6121) supplemented with the XpressAmp™ Solution. N=6.
Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp™ Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp™ Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.
Saliva Direct Protocol for SARS-CoV-2 Surveillance Testing
Evidence of Viral Inactivation by XpressAmp™ Lysis Buffer Video
What’s in the box?
| Item/ Catalog number selected: A8882 | Part # | Size |
|---|---|---|
| XpressAmp™ Lysis Buffer | A886A | 1 × 15ml |
| XpressAmp™ Solution | A887A | 1 × 1.5ml |
| 1-Thioglycerol | A208A | 2 × 75μl |
SDS For A8882
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
| Item/ Catalog number selected: A8880 | Part # | Size |
|---|---|---|
| XpressAmp™ Lysis Buffer | A886B | 1 × 200ml |
| XpressAmp™ Solution | A887B | 1 × 18ml |
| 1-Thioglycerol | A208C | 2 × 1ml |
SDS For A8880
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
