Description
Cut Site:
Câ–ĽTNA Â G
G Â ANTâ–˛C
Notes:
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- DdeI produces an ambiguous extension, which is difficult to ligate.
Incubation Conditions: Buffer D. 37°C.
Source: Desulfovibrio desulfuricans.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 25–50% | 25–50% | 50–75% | 100% | 25–50% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 104 | 97 | 14 | 6 | 29 | 8 |
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
You are viewing:Â R6291 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| DdeI | R629A | 1 Ă— 200u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing:Â R6295 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| DdeI | R629B | 1 Ă— 1,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
