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DdeI

Capable of digesting DNA in 15 minutes or less

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Description

10μ/μl

Catalog number selected: R6291

200u

Catalog number selected: R6291

1,000u

Catalog number selected: R6295

Cut Site:

CTNA  G
G  ANTC

Notes:

  1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  2. DdeI produces an ambiguous extension, which is difficult to ligate.

Incubation Conditions: Buffer D. 37°C.

Source: Desulfovibrio desulfuricans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
25–50% 25–50% 50–75% 100% 25–50%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
104 97 14 6 29 8

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

You are viewing: R6291 Change Configuration
Item Part # Size Concentration Available Separately
Buffer D 10X Buffer R004A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
DdeI R629A 1 × 200u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6295 Change Configuration
Item Part # Size Concentration Available Separately
Buffer D 10X Buffer R004A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
DdeI R629B 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions