Cut Site:

G  CG^▼C
C_▲GC  G

Notes:

1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
2. CfoI is an isoschizomer of HhaI. CfoI is more cost-effective and has 100% activity in MULTI-CORE™ Buffer.

Incubation Conditions: Buffer B. 37°C.

Source: Clostridium formicoaceticum.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.