Description
Cut Site:
A▼GATC T
T CTAG▲A
Usage Note: Ends generated with BglII can be directly ligated to ends generated with BamHI, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Bacillus globigii.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 25–50% | 75–100% | 75–100% | 100% | <10% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 6 | 11 | 0 | 0 | 1 | 0 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6081 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| BglII | R608A | 1 × 500u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6085 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| BglII | R608B | 1 × 2,500u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
