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BglI

  • Genome qualified to ensure optimal performance in genomic analysis applications
  • The 5,000u size is no longer available

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Description

10u/μl

Catalog number selected: R6071

1,000u

Catalog number selected: R6071

Cut Site:

GCCN   NNNNGGC
CGGNNNN   NCCG

Notes:

  1. BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer D. 37°C.

Source: Bacillus globigii.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
10–25% 25–50% 75–100% 100% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
29 20 0 2 1 3

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer D 10X Buffer R004A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
BglI R607A 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions