Description
Convenient RT-PCR in One Tube
Master Mix for Single-Tube RT-PCR
The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases convenience by combining Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer in a single master mix. AMV RT enzyme is provided separately to allow important no-RT control reactions. The master mix is simply added to RNA templates in reaction vials, followed by AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.
- Amplify zeptomole (10–21mol) levels of RNA.
- Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
- System components are stable over many freeze-thaw cycles.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
Protocols
Complete Protocol
What’s in the box?
| Item/ size: 20 reactions Catalog number selected: A1701 | Part # | Size | Available Separately |
| AccessQuick™ Master Mix (2X) | A170A | 2 × 250μl | |
| AMV Reverse Transcriptase | A261B | 1 × 100u | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
SDS For A1701
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
See Protocol for detailed storage recommendations.
What’s in the box?
| Item/ size: 100 reactions Catalog number selected: A1702 | Part # | Size | Available Separately |
| AccessQuick™ Master Mix (2X) | A170B | 3 × 1ml | |
| AMV Reverse Transcriptase | A261A | 1 × 500u | |
| Nuclease-Free Water | P119A | 2 × 1,250μl | View Product |
SDS For A1702
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
See Protocol for detailed storage recommendations.
What’s in the box?
| Item/ size: 500 reactions Catalog number selected: A1703 | Part # | Size | Available Separately |
| AccessQuick™ Master Mix (2X) | A170C | 1 × 12.5ml | |
| AMV Reverse Transcriptase | A261C | 1 × 2,500u | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
SDS For A1703
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents & Disclaimers
Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.
