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Access RT-PCR System

Access RT-PCR System

  • All the reagents you need for single-tube reverse transcription and PCR
  • Separate components allow you to control every step of the reaction

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Description

Size

Catalog number selected:  A1260

Catalog number selected: A1250

Catalog number selected: A1280

Convenient RT-PCR in One Tube

 

 

 

 

Separate Components to Assemble Your Single Tube RT-PCR

The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples. In addition, the improved performance of AMV Reverse Transcriptase at elevated temperatures in the AMV/Tfl 5X Reaction Buffer minimizes problems encountered with secondary structures in RNA.

  • Maximum Control: Separate tubes of each component allow you to control every step of the reaction. You can optimize Mg2+ and perform no-reverse transcriptase control reactions.
  • Less Template: Detect message from as little as 1pg of total RNA or mRNA.
  • No Buffer Additions Required: The AMV/Tfl 5X Reaction Buffer results in optimal enzyme activity without buffer additions between the reverse transcription and DNA amplification steps.
  • Performance-Tested System: Promega PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

For more information, see the Protocols & Applications Guide.

  1. Miller, K. and Storts, D.R. (1995) Promega Notes 53, 2–5.
  2. Kaledin, A.S. et al. (1981) Biokhimiia 46, 1576–84.

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Performance Guarantee:
 Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Protocols

Complete Protocol

Access RT-PCR System Technical Bulletin
Download

Quick Protocols

Access RT-PCR system Quick Protocol FB034
Download

Specifications

What’s in the box?

  Item/ size: 20 reactions Catalog number selected: A1260 Part # Size Concentration Available Separately
Upstream Control Primer A109B 1 × 100μl 15μM
Downstream Control Primer A110B 1 × 100μl 15μM
Positive Control RNA with Carrier A111A 1 × 50μl 62.5amol
AMV Reverse Transcriptase A261B 1 × 100u
AMV/Tfl 5X Reaction Buffer A808A 1 × 1ml
dNTP Mix C114F 1 × 20μl 10mM
MgSO4 M197A 1 × 1,250μl 25mM
Tfl DNA Polymerase M391A 1 × 100u
Nuclease-Free Water P119E 1 × 13ml View Product

SDS For A1260

Download SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

  Item/ size: 100 reactions Catalog number selected: A1250 Part # Size Concentration Available Separately
Upstream Control Primer A109B 1 × 100μl 15μM
Downstream Control Primer A110B 1 × 100μl 15μM
Positive Control RNA with Carrier A111A 1 × 50μl 62.5amol
AMV Reverse Transcriptase A261A 1 × 500u
AMV/Tfl 5X Reaction Buffer A808A 1 × 1ml
dNTP Mix C114A 2 × 50μl 10mM
MgSO4 M197A 1 × 1,250μl 25mM
Tfl DNA Polymerase M391C 1 × 500u
Nuclease-Free Water P119E 1 × 13ml View Product

SDS For A1250

Download SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

What’s in the box?

  Item/ size: 500 reactions Catalog number selected: A1280 Part # Size Concentration Available Separately
Upstream Control Primer A109B 1 × 100μl 15μM
Downstream Control Primer A110B 1 × 100μl 15μM
Positive Control RNA with Carrier A111A 1 × 50μl 62.5amol
Access RT-PCR Core Reagents (100 reactions; A1250 minus Controls) A1281 4 × 1 each
AMV Reverse Transcriptase A261A 1 × 500u
AMV/Tfl 5X Reaction Buffer A808A 1 × 1ml
dNTP Mix C114A 2 × 50μl 10mM
MgSO4 M197A 1 × 1,250μl 25mM
Tfl DNA Polymerase M391C 1 × 500u
Nuclease-Free Water P119E 1 × 13ml View Product

SDS For A1280

Download SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents & Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.