DNA Step Ladders have defined sizes with exact incremental steps between bands. The ladders are not intended for use in quantitative analysis. The DNA fragments may be stained with ethidium bromide.

The 50bp DNA Step Ladder has 16 DNA fragments ranging from 50bp to 800bp in 50bp increments plus an 1,800bp “backbone” fragment. All bands except the 800bp band are of equal intensity; the 800bp band is ≈3 times more intense.

Storage Buffer: 10mM Tris-HCl (pH 7.5), 1mM EDTA.