DNA Ladders have defined sizes, and are not intended for use in quantitative analysis.
The 100bp DNA Ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 500bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase for visualization by autoradiography.
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