Nucleic Acid Amplification

Nucleic Acid Amplification

Nucleic Acid Amplification

After you’ve purified and quantitated your nucleic acid, the next step is amplification. PCR is perhaps the most ubiquitous assay in molecular biology—something we do every day. Each type of PCR has different applications and comes with its own unique challenges. Which one is right for your project?

Endpoint PCR

Endpoint PCR was originally developed to amplify short segments of a longer DNA molecule. It is a relatively simple application that is used to exponentially increase the number of copies of a given DNA or cDNA template. 


In a real-time quantitative PCR reaction (qPCR), product formation is monitored following each amplification cycle, as opposed to endpoint PCR, which only measures after all cycles are complete. This application is a part of nearly every recently published study, and is generally regarded as invaluable.

Molecular Biology Lab Guide

From basic DNA purification to the most complicated qPCR experiment, the Molecular Biology Lab Guide can help you make every experiment successful.

Learn More »