- Review current DNA/RNA quantitation methods, including absorbance, fluorescent nucleic acid-binding dyes and qPCR
- Learn about the advantages and disadvantages of each method
Summary
Accurate and reliable nucleic acid quantitation is a critical step in DNA and RNA analysis workflows. Often success or failure in downstream applications simply comes down to whether or not the appropriate amount of input nucleic acid is used. There are a number of key considerations when quantifying nucleic acids with the goal being to obtain not only an accurate measurement of yield/concentration, but often integrity and purity as well. Not all quantitation methods reveal the same information about a sample, so understanding the strengths and weaknesses of each method allows a researcher to choose an appropriate method based on the starting sample type and downstream assay. This webinar will review current quantitation methods including absorbance, fluorescent nucleic acid-binding dyes and qPCR. The advantages and disadvantages of each method will be discussed including data from samples that were quantitated using multiple methods to highlight the differences in these methods. Ultimately, no single quantitation method is ideal for every situation, and after this webinar, you will be able to choose the appropriate method required for downstream application success.
Speaker
Douglas Wieczorek, PhD
Senior Applications Scientist
Dr. Doug Wieczorek is a Senior Applications Scientist at Promega Corporation, Madison, WI. Dr. Wieczorek started his career at Promega in the Technical Services department before moving to the Scientific Applications group, which focuses on the development of new applications for existing products in response to customer requests. Prior to joining Promega, he received his Ph.D. in 2001 from the Interdisciplinary Graduate Program in Genetics at the University of Iowa where he studied viral and bacteriophage DNA packaging. He followed his thesis work with postdoctoral training in the Department of Microbiology at the University of Iowa as well as the Department of Genetics at the University of Wisconsin – Madison.