The Wizard® SV 96 PCR Clean-Up System is designed for high-throughput purification of 100bp to 10kb PCR products from excess nucleotides, primers and primer dimers. Average recovery is >90% for 100–500bp fragments, and purification is complete in as little as 20 minutes. The product requires use of a vacuum pump and 96-well vacuum manifold (Vac-Man® 96 Vacuum Manifold, Cat.# A2291).

The purified DNA can be used for automated fluorescent sequencing (Phred* 20 scores >600), cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard® SV 96 PCR Clean-Up System uses 96-well filtration without the need to disassemble the manifold. Filtrate waste is delivered directly to a vacuum trap, eliminating the need to dispose of collected waste within the manifold assembly.

*A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.

Protocols