Simple, Reliable Plasmid Purification

The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less.  The Vac-Man® 96 Vacuum Manifold eliminates waste handling and allows simultaneous lysate clearing and DNA binding, and the plate design prevents cross-contamination during sample processing.

Purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. Purified DNA meets a target of >98% accuracy over 600 bases using pGEM®-3Zf(+) Vector DNA in BigDye® terminator sequencing.

The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).