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SmaI

  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

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Description

8-12u/μl

Catalog number selected: R6121

1,000u

Catalog number selected: R6121

5,000u

Catalog number selected: R6125

Cut Site:

CCCGGG
GGGCCC

Note: XmaI is a neoschizomer of SmaI.

Incubation Conditions: Buffer J. 25°C. (SmaI exhibits 25–50% activity at 37°C.)

Source: Serratia marcescens.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D J MULTI-CORE™
<10% <10% <10% <10% 100% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
3 12 0 1 1 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

You are viewing: R6121 Change Configuration
Item Part # Size Concentration Available Separately
Buffer J 10X Buffer R009A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
SmaI R612A 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing:  R6125 Change Configuration
Item Part # Size Concentration Available Separately
Buffer J 10X Buffer R009A 1 × 1ml
Bovine Serum Albumin, Acetylated R396E 1 × 750μl 10mg/ml View Product
SmaI R612F 1 × 5,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions