Description
Cut Site:
G CATG▼C
C▲GTAC G
Incubation Conditions: Buffer K. 37°C.
Source: Streptomyces phaeochromogenes.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 100mM sodium citrate, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | K | MULTI-CORE™ |
| 75–100% | 75–100% | 100%* | 75–100% | 100% | 10–25% |
| *Buffer C is not recommended due to potential star activity. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 6 | 8 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6261 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer K 10X Buffer | R010A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| SphI | R626A | 1 × 200u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6265 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer K 10X Buffer | R010A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| SphI | R626B | 1 × 1,000u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
