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SgfI

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Description

8-12μ/μl

Catalog number selected:  R7103

250u

Catalog number selected:  R7103

Cut Site:

GCG  ATCGC
CGCTA  GCG

Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. Sensitive to CpG methylation.
  3. SgfI is inactivated by freezing at –70°C.
  4. Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Incubation Conditions: Buffer C. 37°C.

Source: Streptomyces fulvissimis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D K MULTI-CORE™
25–50% 25–50% 25–50% 50–75% 100% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
13 5 0 0 0 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer C 10X Buffer R003A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
SgfI R710B 1 × 250u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions