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RsaI

  • Capable of digesting DNA in 15 minutes or less
  • High concentration product no longer available

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Description

10μ/μl

Catalog number selected: R6371

1,000u

Catalog number selected: R6371

Cut Site:

GTAC
CATG

Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer C. 37°C.

Source: Rhodopseudomonas sphaeroides.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
75–100% 75–100% 100% <10% <10%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
113 83 11 3 19 3

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer C 10X Buffer R003A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
RsaI R637A 1 × 1,000u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions