Description
RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])
Applications
- Preparation of DNA-free RNA.
- Degradation of DNA from RNA transcription systems.
- Nick translation of DNA.
- Studying DNA:protein interactions by DNase I footprinting.
Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.
Source: Bovine pancreas.
QC Tests: Activity, RNase.
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.
Protocols
What’s in the box?
Size: 1,000u
| Item / Catlog no: M6101 | Part # | Size | Available Separately |
| RQ1 DNase 10X Reaction Buffer | M198A | 1 × 1ml | RQ1 DNase 10X Reaction Buffer |
| RQ1 DNase Stop Solution | M199A | 1 × 1ml | RQ1 DNase Stop Solution |
| RQ1 RNase-Free DNase | M610A | 1 × 1,000u | RQ1 RNase-Free DNase |
SDS
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
