Description
The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.
Applications of the Riboprobe® Systems include:
- Generation of high-specific-activity RNA probes.
- Generation of microgram quantities of RNA transcripts.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
Protocols
What’s in the box?
| Item / Catalog number: P1420 | Part # | Size | Concentration | Available Separately |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product | |
| Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
| SP6 RNA Polymerase | P108E | 1 × 500u | View Product | |
| rATP | P113A | 1 × 50μl | 10mM | View Product |
| rCTP | P114A | 1 × 50μl | 10mM | View Product |
| rGTP | P115A | 1 × 50μl | 10mM | View Product |
| rUTP | P116A | 1 × 50μl | 10mM | View Product |
| DTT | P117A | 1 × 100μl | 100mM | View Product |
| Transcription Optimized 5X Buffer | P118B | 1 × 500μl | View Product | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product | |
| pGEM® Express Positive Control Template | P256A | 1 × 5μg | View Product | |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item / Catalog number: P1430 | Part # | Size | Concentration | Available Separately |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product | |
| Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
| rCTP | P114A | 1 × 50μl | 10mM | View Product |
| rATP | P113A | 1 × 50μl | 10mM | View Product |
| rGTP | P115A | 1 × 50μl | 10mM | View Product |
| rUTP | P116A | 1 × 50μl | 10mM | View Product |
| DTT | P117A | 1 × 100μl | 100mM | View Product |
| Transcription Optimized 5X Buffer | P118B | 1 × 500μl | View Product | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product | |
| T3 RNA Polymerase | P208A | 1 × 500u | View Product | |
| pGEM® Express Positive Control Template | P256A | 1 × 5μg | View Product | |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item / Catalog number: P1440 | Part # | Size | Concentration | Available Separately |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product | |
| Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
| rATP | P113A | 1 × 50μl | 10mM | View Product |
| rCTP | P114A | 1 × 50μl | 10mM | View Product |
| rGTP | P115A | 1 × 50μl | 10mM | View Product |
| rUTP | P116A | 1 × 50μl | 10mM | View Product |
| DTT | P117A | 1 × 100μl | 100mM | View Product |
| Transcription Optimized 5X Buffer | P118B | 1 × 500μl | View Product | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product | |
| T7 RNA Polymerase | P207D | 1 × 500u | View Product | |
| pGEM® Express Positive Control Template | P256A | 1 × 5μg | View Product | |
| RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
