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Reverse Transcription System

Reverse Transcription System

Convenient System for Reverse Transcription Using AMV Reverse Transcriptase

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Description

Size

Catalog number selected: A3500

Convenient System for Reverse Transcription Using AMV Reverse Transcriptase

 

 

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.

  • Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes.
  • Convenience: PCR-compatible components are provided in optimized volumes for 100 reactions.
  • Positive Controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters.

Protocols

Complete Protocol

Reverse Transcription System Technical Bulletin
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Quick Protocol

Reverse Transcription System Quick Protocol FB039
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Specifications

What’s in the box?

  Item/ Catalog number selected: A3500 Part # Size Concentration Available Separately
MgCl2 A351H 1 × 1.2ml 25mM View Product
Reverse Transcription 10X Buffer A356A 1 × 1.4ml View Product
Oligo(dT)15 Primer C110B 1 × 50μg 500μg/ml View Product
dNTP Mix C114B 1 × 320μl 10mM View Product
Random Primers C118B 1 × 50μg View Product
1.2kb Kanamycin Positive Control RNA C138A 1 × 5μg 0.5mg/ml View Product
AMV Reverse Transcriptase (HC) M900B 1 × 1,500u View Product
Recombinant RNasin® Ribonuclease Inhibitor N251A 1 × 2,500u 40u/μl View Product
Nuclease-Free Water P119E 1 × 13ml View Product

SDS For  A3500

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.