Description
Concentrate RNA from large volumes to ≤15µl
Prepare RNA Samples for Downstream Applications
The ReliaPrep™ RNA Clean-Up and Concentration System was designed to reduce carryover of reagents and concentrate dilute samples in a simple, fast protocol. The system can process dilute samples or sample pools up to 300µl in a single column and elute them in 15µl of water or TE buffer. It maintains good recovery of RNA samples and ensures usefulness in downstream applications such as RT-qPCR, Northern blot analysis or RNA sequencing.
Five micrograms of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 79.8%.
One microgram of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 74.9%.
Protocols
What’s in the box ?
| Item - Catalog number: Z1071 | Part # | Size | Available Separately |
| Collection Tubes | A130C | 1 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| Column Wash Solution (CWE) | A254A | 1 × 1ml | |
| Membrane Binding Solution | A930A | 1 × 4ml | View Product |
| Elution Tubes | Z200A | 2 × 5/pk | |
| RNA Wash Solution (RWA) | Z309B | 1 × 11.8ml | View Product |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
without Vacuum Adapters
| Item - Catalog number: Z1072 | Part # | Size | Available Separately |
| Column Wash Solution (CWE) | A254B | 1 × 5ml | |
| Membrane Binding Solution | A930B | 1 × 20ml | View Product |
| RNA Wash Solution (RWA) | Z309B | 1 × 11.8ml | View Product |
| ReliaPrep™ Minicolumns | A207B | 1 × 50/pack | |
| Collection Tubes | A130A | 1 × 50/pk | |
| Elution Tubes | Z200B | 2 × 25/pk | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box ?
| Item - Catalog number: Z1073 | Part # | Size | Available Separately |
| Column Wash Solution (CWE) | A254C | 1 × 24ml | |
| Membrane Binding Solution | A930B | 2 × 20ml | View Product |
| Elution Tubes | Z200C | 5 × 50/pk | |
| RNA Wash Solution (RWA) | Z3091 | 1 × 58.8ml | |
| ReliaPrep™ Minicolumns | A207B | 5 × 50/pack | |
| Collection Tubes | A130B | 1 × 250/pk | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
