Description
Sensitive Quantitation of Small Amounts of RNA in Solution
Highly Sensitive, Easy-to-Use, Fluorescent Dye for RNA Quantitation
The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is important for many biological applications, including determining yield of in vitro transcribed RNA and measuring RNA concentration before Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, RT-PCR and differential display PCR.
The QuantiFluor® System requires less RNA (100pg) than other RNA quantitation methods (~1µg), so you save more of your sample for downstream assays.
Improved Sensitivity vs. Absorbance and Other Fluorescent RNA Quantitation Systems
The system is designed to measure 0.1–500ng/µl RNA (from 1µl sample in a 200µl assay) and offers significantly increased sensitivity over absorbance at 260nm (NanoDrop) for samples containing low concentrations of RNA. Sensitivity is equivalent to that of Quant-iT™ RiboGreen®, with wider dynamic range.
| Sample* | Assay | |
| QuantiFluor® RNA System | 0.1–500ng/µl | 0.1–500ng |
| Quant-iT™ RiboGreen | 0.1–200ng/µl | 0.1–200ng |
| Qubit® RNA HS (5–100ng) | 5–100ng/µl | 5–100ng |
| NanoDrop® 2000 Spectrophotometer | 2–12,000ng/µl | 2–12,000ng |
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input RNA per assay.
RNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® RNA System detects as little as 0.5ng/ml RNA in a 96-well microplate assay (corresponding to 1µl of a 100ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
What’s in the box?
| Item Catlog number: E3310 | Part # | Size |
| 20X TE Buffer (pH 7.5) | E260A | 1 × 25ml |
| RNA Standard | E299A | 1 × 100μg |
| QuantiFluor® RNA Dye | E286A | 1 × 1ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
