Description
Sensitive Quantitation of Double-Stranded DNA in Solution
Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.
The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, “Add-and-Read” format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It’s as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples.
Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems
The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).
| Sample* | Assay | |
| Quantifluor® ONE dsDNA System | 0.2–400ng/µl | 0.2–400ng |
| Qubit® dsDNA HS | 0.2–100ng/µl | 0.2–100ng |
| Qubit® dsDNA BR | 2–1,000ng/µl | 2–1,000ng |
*Based on 1µl sample input per assay.
DNA Quantitation Assay Sensitivity and Linearity
The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
What’s in the box?
| Item | Part # | Size |
|---|---|---|
| QuantiFluor® ONE dsDNA Dye | E489A | 1 × 20ml |
| QuantiFluor® ONE Lambda DNA | E490B | 1 × 80μg |
| 1X TE Buffer (pH 7.5) | E497B | 1 × 10ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item | Part # | Size |
|---|---|---|
| QuantiFluor® ONE dsDNA Dye | E489A | 5 × 20ml |
| QuantiFluor® ONE Lambda DNA | E490A | 1 × 400μg |
| 1X TE Buffer (pH 7.5) | E497A | 1 × 25ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
