Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.
The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, “Add-and-Read” format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It’s as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples.
Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems
The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).
|Quantifluor® ONE dsDNA System||0.2–400ng/µl||0.2–400ng|
|Qubit® dsDNA HS||0.2–100ng/µl||0.2–100ng|
|Qubit® dsDNA BR||2–1,000ng/µl||2–1,000ng|
DNA Quantitation Assay Sensitivity and Linearity
The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
What’s in the box?
|QuantiFluor® ONE dsDNA Dye||E489A||1 × 20ml|
|QuantiFluor® ONE Lambda DNA||E490B||1 × 80μg|
|1X TE Buffer (pH 7.5)||E497B||1 × 10ml|
For Research Use Only. Not for Use in Diagnostic Procedures.
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.