Description
Size:
2ml : Catalog number selected: A6101
10ml: Catalog number selected: A6102
Size:
2ml : Catalog number selected: A6120
12.5ml: Catalog number selected: A6121
Size:
2ml : Catalog number selected: A6110
Simple, Sensitive Probe-Based qPCR and RT-qPCR
The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These real-time qPCR systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.
Rapid hot-start activation and processive enzymes make the GoTaq® Probe qPCR and RT-qPCR Systems compatible with most real-time PCR instruments using standard or fast cycling. Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.
Choose qPCR or RT-qPCR Master Mix Formats
Sensitivity and Specificity for qPCR
The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoTaq® Probe qPCR Master Mix offers:
- Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
- Optimized formulation compatible with running multiplex assays.
- Room-temperature setup, making it suitable for automation and high-throughput detection.
- Resistance to a wide range of PCR inhibitors.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6101 | 2ml | 400 reactions | 200 reactions |
| A6102 | 10ml | 2,000 reactions | 1,000 reactions |
qPCR amplification from FFPE samples with GoTaq® Probe Master Mix. DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Reverse Transcription and qPCR in One Reaction
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6120 | 2ml | 400 reactions | 200 reactions |
| A6121 | 12.5ml | 2,500 reactions | 1,250 reactions |
Expression levels for the Zmm19 gene in corn. Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).
Two-Step Reverse Transcription and qPCR
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6110 | 2ml | 50 × 20µl RT reactions and 400 × 10µl qPCR reactions | 50 × 20µl RT reactions and 200 × 20µl qPCR reactions |
Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.
Suitable for Different Organisms and Sample Types
DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
Organism Types:
- Animal
- Bacteria
- Fungi
- Human
- Parasites
- Viruses
- Yeast
Sample Types:
- Blood
- Cells
- Feces
- FFPE
- Food
- Human Cerebrospinal Fluid (CSF)
- Plants
- Plasma
- Saliva
- Serum
- Sputum
- Swabs
- Tissue
- Urine
Compatible with these Instruments
For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
- Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
- Applied Biosystems 7300 and 7900HT Real-Time PCR System
- Applied Biosystems GeneAmp® 5700 Thermal Cycler
- Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
- Applied Biosystems ViiA® 7 Real-Time PCR System
- Applied Biosystems QuantStudio® Real Time PCR Systems
- Bio-Rad CFX96 Real-Time PCR Detection System
- Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
- Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
- Bio-Rad/MJ Research Chromo4™ Real-Time Detector
- Bio-Rad MyiQ™ Real-Time PCR Detection System
- Cepheid SmartCycler® System
- Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
- Eppendorf Mastercycler® ep realplex Real-Time PCR System
- Roche LightCycler® 480 Real-Time PCR System
- Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
- Stratagene Mx4000® Multiplex Quantitative PCR System
Protocols
What’s in the box?
| Item/ size 2ml : Catalog number selected: A6101 | Part # | Size | Available Separately | Concentration |
| GoTaq® Probe qPCR Master Mix, 2X | A610A | 2 × 1ml | ||
| CXR Reference Dye | C541A | 1 × 100μl | View Product | |
| Nuclease-Free Water | P119A | 2 × 1,250μl | View Product | |
| Item/ size 10ml: Catalog number selected: A6102 | Part # | Size | Available Separately | Concentration |
| GoTaq® Probe qPCR Master Mix, 2X | A610A | 10 × 1ml | ||
| CXR Reference Dye | C541B | 2 × 200μl | View Product | 30μM |
| Nuclease-Free Water | P119E | 1 × 13ml | View Product |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item/ size 2ml : Catalog number selected: A6120 | Part # | Size | Concentration | Available Separately |
| GoTaq® Probe Master Mix with dUTP | A612A | 2 × 1ml | ||
| CXR Reference Dye | C541B | 1 × 200μl | 30μM | View Product |
| GoScript™ RT Mix for 1-Step RT-qPCR | M700A | 1 × 225μl | 50X | |
| Nuclease-Free Water | P119A | 2 × 1,250μl | View Product | |
| Item/ size 12.5ml: Catalog number selected: A6121 | Part # | Size | Concentration | Available Separately |
| GoTaq® Probe Master Mix with dUTP | A612B | 1 × 12.5ml | ||
| CXR Reference Dye | C541C | 1 × 500μl | 30μM | View Product |
| GoScript™ RT Mix for 1-Step RT-qPCR | M700C | 1 × 500μl | ||
| Nuclease-Free Water | P119E | 1 × 13ml | View Product |
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
| Item/ size 2ml : Catalog number selected: A6110 | Part # | Size | Concentration | Available Separately |
| MgCl2 | A351B | 1 × 750μl | 25mM | View Product |
| GoScript™ 5X Reaction Buffer | A500B | 1 × 300μl | View Product | |
| GoScript™ Reverse Transcriptase | A501B | 1 × 50μl | ||
| GoTaq® Probe qPCR Master Mix, 2X | A610A | 2 × 1ml | ||
| Oligo(dT)15 Primer | C110B | 1 × 50μg | 500μg/ml | View Product |
| PCR Nucleotide Mix | C114G | 1 × 200μl | 10mM | View Product |
| Random Primers | C118B | 1 × 50μg | View Product | |
| CXR Reference Dye | C541A | 1 × 100μl | View Product | |
| Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
| Nuclease-Free Water | P119A | 3 × 1,250μl | View Product |
SDS For A6110
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
