Description
Cut Site:
CG AT▼CG
GC▲TA GC
Usage Notes:
- Religation of DNA fragments with dam methylation is difficult.
- Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
Incubation Conditions: Buffer D. 37°C.
Source: Proteus vulgaris.
Storage Buffer: 10mM Tris-HCl (pH 7.3 @ 25°C), 50mM NaCl, 0.1% Triton® X-100, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 10–25% | 25–50% | 50–75% | 100% | <10% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 3 | 7 | 0 | 2 | 1 | 1 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6321 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| PvuI | R632A | 1 × 100u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6325 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer D 10X Buffer | R004A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| PvuI | R632D | 1 × 500u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
