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pSP73 Vector

pSP73 Vector

Standard Cloning Vector That Also Transcribes RNA In Vitro

  • Versatile vector that can be used for standard cloning and in vitro transcription
  • SP6 and T7 RNA polymerase promoters flank the multiple cloning region
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

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Description

Size: 20µg
Catalog number: P2221

The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.

Reference

  1. Krieg, P.A. and Melton, D.A. (1987) Meth. Enzymol. 155, 397–415.
0300VA05_2A-W

pSP73 Vector GenBank® Accession Number X65333 and vector sequence text file.

Protocols

Complete Protocol

pSP73 Vector Technical Bulletin


Specifications

What’s in the box?
Size: 20µg

Item Part # Size
pSP73 Vector P222A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB