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Prime-a-Gene® Labeling System

Prime-a-Gene® Labeling System

Complete Kit for Random-Primed Labeling of Linear Template DNA, Excluding Radionucleotides

  • Ready-to-use reagents for random-primed labeling of linear DNA, including random hexamer primers (excludes radionucleotides)
  • Probes generated with high specific activities >1 × 109cpm/μg

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Description

Size

The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers, for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 109cpm/μg.

Applications

  • Detection of single-copy genes and rare sequences.
  • Southern, Northern and dot blotting.
  • Plaque and colony lift hybridizations.

Protocols

Complete Protocol

Prime-a-Gene® Labeling System Technical Bulletin


Specifications

What’s in the box?

Item / Catlog no: U1100 Part # Size Available Separately
DNA Polymerase I Large (Klenow) Fragment M220A 1 × 150u View Product
Nuclease-Free Water P119A 1 × 1,250μl View Product
Prime-a-Gene® dATP U111A 1 × 40μl
Prime-a-Gene® dCTP U112A 1 × 40μl
Prime-a-Gene® dGTP U113A 1 × 40μl
Prime-a-Gene® dTTP U114A 1 × 40μl
Labeling 5X Buffer U115A 1 × 300μl View Product
BSA, Nuclease-Free U116A 1 × 75μl
Prime-a-Gene® Control DNA U117A 1 × 25μl

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB