Description
The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers, for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 109cpm/μg.
Applications
- Detection of single-copy genes and rare sequences.
- Southern, Northern and dot blotting.
- Plaque and colony lift hybridizations.
Protocols
Complete Protocol
Prime-a-Gene® Labeling System Technical Bulletin
What’s in the box?
Item / Catlog no: U1100 | Part # | Size | Available Separately |
DNA Polymerase I Large (Klenow) Fragment | M220A | 1 × 150u | View Product |
Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
Prime-a-Gene® dATP | U111A | 1 × 40μl | |
Prime-a-Gene® dCTP | U112A | 1 × 40μl | |
Prime-a-Gene® dGTP | U113A | 1 × 40μl | |
Prime-a-Gene® dTTP | U114A | 1 × 40μl | |
Labeling 5X Buffer | U115A | 1 × 300μl | View Product |
BSA, Nuclease-Free | U116A | 1 × 75μl | |
Prime-a-Gene® Control DNA | U117A | 1 × 25μl |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions