Description
The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19
pGEM®-3Z Vector GenBank® Accession Number X65304 and vector sequence text file.
Protocols
What’s in the box?
| Item | Part # | Size |
|---|---|---|
| pGEM®-3Z Vector | P215A | 1 × 20μg |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
