Description
GATC
CTAGâ–˛
Notes:
- Sensitive to dam methylation.
- Sau3AI is an isoschizomer of MboI that is not sensitive to dam methylation.
- DpnI is an unusual isoschizomer of MboI that will cut only if the recognition site is methylated by the dam methylation system.
Incubation Conditions: Buffer C. 37°C.
Source: Moraxella bovis ATCC 10900.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM KCl, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 10–25% | 75–100% | 100% | 50–75% | <10% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 16 | 87 | 0 | 15 | 6 | 22 |
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer C 10X Buffer | R003A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| MboI | R671A | 1 Ă— 200u |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
