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MboI

Description

8-12μ/μl

Catalog number selected: R6711

500u

Catalog number selected: R6711

GATC
CTAG

Notes:

  1. Sensitive to dam methylation.
  2. Sau3AI is an isoschizomer of MboI that is not sensitive to dam methylation.
  3. DpnI is an unusual isoschizomer of MboI that will cut only if the recognition site is methylated by the dam methylation system.

Incubation Conditions: Buffer C. 37°C.

Source: Moraxella bovis ATCC 10900.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM KCl, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
10–25% 75–100% 100% 50–75% <10%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
16 87 0 15 6 22

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer C 10X Buffer R003A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
MboI R671A 1 × 200u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions