Description
Automated Purification of RNA from Whole Blood using a Low Elution Volume
Benchtop RNA Purification from Blood Samples
The Maxwell® RSC simplyRNA Blood Kit, used with the Maxwell® RSC Instruments, provides a simple method for automated RNA purification from 2.5ml of fresh whole blood. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The kit provides the necessary reagents for processing the samples and uses prefilled cartridges for purification, maximizing simplicity and convenience. The Maxwell® RSC Instrument can process from 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process from 1 to 48 samples, in about 50 minutes.
Applications
RNA extraction from blood can be used in quantitative RT-PCR, gene expression and translation assays.
Maxwell® Multi-Packs
The Maxwell® Multi-Pack (Cat.# ASB1380) provides the Maxwell® Plungers in a 50/pk bag format and is not compatible with the Maxprep™ Liquid Handler for automated sample preprocessing.
Protocols
What’s in the box?
| Item - Catalog number: AS1380 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Cell Lysis Solution | A793A | 4 × 100ml | View Product |
| Maxwell® RSC Cartridge (RSCE) | AS138A | 48 × 1 each | |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Proteinase K (PK) Solution | MC500C | 2 × 1ml | View Product |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 2 × 1 vial | |
| RSC Plungers | AS1670 | 1 × 48/pack | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item - Catalog number: ASB1380 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B-C | 3 × 900μl | |
| Cell Lysis Solution | A793A | 12 × 100ml | View Product |
| Maxwell® RSC Cartridge (RSCE) | AS138A | 144 × 1 each | |
| Elution Tubes (0.5ml) | AS620A | 3 × 50/pk | View Product |
| Blue Dye | C888A | 3 × 50μl | |
| Proteinase K (PK) Solution | MC500C | 6 × 1ml | View Product |
| Lysis Buffer | MC501C | 3 × 20ml | |
| Nuclease-Free Water | P119C-C | 3 × 25ml | |
| Homogenization Solution | Z305H-C | 3 × 30ml | |
| DNase I (lyophilized) | Z358A-C | 6 × 1 vial | |
| CSC/RSC Plungers | AS133A | 3 × 50/pk |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. No. 6,855,499 and other patents.
