Description
Automated Purification of High-Quality Total RNA with an Enhanced miRNA Enrichment
Purify High-Quality miRNA from Tissue, Plasma, Serum or Enriched Exosomes
The Maxwell® RSC miRNA Tissue Kit purifies total RNA, including microRNA (miRNA), from 1–48 tissue samples in about 90 minutes. The Maxwell® RSC miRNA Plasma and Serum Kit will purify total RNA, including miRNA, from 1–48 samples in about 70 minutes. The kits provide all the reagents required for sample processing and use prefilled cartridges for purification, simplifying extraction and freeing up your time for analysis.
The Maxwell® RSC miRNA Tissue and miRNA Plasma and Serum Kits work with both the Maxwell® RSC and RSC 48 instruments. The Maxwell® RSC Instrument can process from 1 to 16 samples and the Maxwell® RSC 48 can process from 1 to 48 samples in a single run. The Maxwell® RSC miRNA Tissue and miRNA Plasma and Serum Kits are also compatible for use on the Maxwell® CSC Instrument in RUO Mode.
The extracted RNA is highly concentrated and ready for use in RT-qPCR, RNA sequencing and other downstream applications used in biomarker detection, gene expression and other studies.
No clogging worries with a paramagnetic particle mover
Because the Maxwell® RSC Instruments are magnetic particle movers, not liquid handlers, they offer advantages over other automated systems. There is minimal risk of cross-contamination because no liquid handling of splattering happens during sample processing. With no clogs and fewer breakdowns, there are fewer disruptions in your workflow.
Using tissue
Using plasma, serum or enriched exosomes
miRNA from tissue
Multiple miRNA targets can be consistently purified from mammalian tissues. The high-quality RNA is ready to use in downstream amplification assays such as RT-qPCR.
miRNA purified from pancreas, kidney and liver samples. miRNA yield was quantitated for both mir-21 and let-7a targets by RT-qPCR using TaqMan®-based miRNA-specific primers and standards.
miRNA from plasma
Consistent purification of high-quality miRNA from plasma, serum or enriched exosomes.
Protocols
Complete Protocol
Quick Protocols
What’s in the box?
Size: 48 preps
| Item - Catalog number: AS1680 | Part # | Size | Available Separately |
| RSC Plungers | AS1670 | 1 × 48/pack | View Product |
| Maxwell® RSC Cartridge (RSCL) | AS168A | 48 × 1 each | |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Lysis Buffer C | MC136A | 1 × 25ml | |
| Proteinase K (PK) Solution | MC500C | 5 × 1ml | View Product |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| DNase I (lyophilized) | Z358A | 2 × 1 vial |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 48 preps
| Item - Catalog number: AS1460 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Maxwell® RSC Cartridge (RSCN) | AS173A | 48 × 1 each | |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Proteinase K (PK) Solution | MC500C | 2 × 1ml | View Product |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 2 × 1 vial | |
| RSC Plungers | AS1670 | 1 × 48/pack | View Product |
| Lytic Enhancer | MC145A | 1 × 20ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.
