Description
Reliable, Automated DNA Purification from Fecal Samples
Simple, Reproducible DNA Extraction from Fecal Samples
The Maxwell® RSC Fecal Microbiome DNA Kit used with the Maxwell® RSC Instruments provides a simple method for efficient, automated isolation of nucleic acid from human and mammalian fecal samples.
Fecal microbiome characterization is a rapidly evolving field, with continued development of downstream assays. Complex analyses such as next-generation sequencing can enhance data quality by measuring the abundance of various bacterial species. Many extraction methods for fecal microbiome DNA involve several manual processing steps and mechanical disruption. The Maxwell® RSC Fecal Microbiome DNA Kit purifies microbiome DNA for characterization in downstream assays including qPCR and next-generation sequencing.
Advantages
- Simple, automated protocol requires minimal hands-on steps.
- No organic extractions or laborious sample preprocessing steps.
- Optimized to extract microbial DNA from fecal samples for metagenomic analysis.
A quick protocol for fecal DNA extraction without the need for bead beating.
DNA from 100mg and 300mg of human fecal samples was extracted using the Maxwell® RSC Fecal Microbiome DNA Kit. Yield and concentration of extracted DNA was determined using the QuantiFluor® dsDNA System (Cat.# E2670, E2671).
Protocols
What’s in the box?
| Item/ Catalog number selected: AS1700 | Part # | Size | Concentration | Available Separately |
| Maxwell® RSC Cartridge (RSCJ) | AS159A | 48 × 1 each | ||
| Proteinase K (PK) Solution | MC500C | 2 × 1ml | View Product | |
| Lysis Buffer | MC141A | 1 × 100ml | ||
| RNase A Solution | A797C | 1 × 1ml | 4mg/ml | View Product |
| Elution Buffer | A828D | 1 × 20ml | View Product | |
| Binding Buffer | MC501D | 1 × 20ml | ||
| RSC Plungers | AS1670 | 1 × 48/pack | View Product | |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
SDS For AS1700
Download SDSUse Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents & Disclaimers
U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.
